Composite

Part:BBa_K4194014:Design

Designed by: Jiong Huang   Group: iGEM22_BUCT-China   (2022-09-24)


p(Bla)-RBS-(c-di-GMP riboswitch)-mRFP1-LVA-RBS-TetR-LVA-terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 838
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 720
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 141


Design Notes

Codon optimization for Escherichia coli has been done.


Source

None

References